Читать книгу Genotyping by Sequencing for Crop Improvement - Группа авторов - Страница 66

Vitis is native to temperate regions and having 30 wild species indigenous to East Asia and 28 wild species from North America. Liang and coworkers resequenced the whole genome of 472 Vitis accessions that cover 48 out of the 60 extant species from different geographical distributions. They had generated around 4.1 Tb WGS data, 27.3 billion paired‐end raw reads for 472 accessions. For the majority of accessions, the coverage was found more than 80%. By using different filter criteria, they identified the 77 726 929 SNPs, 10 278 017 indels, and 23K copy number of the variants. Next filtering yielded 27 859 960 SNPs, 3 854 659 indels with minor allele frequency (MAF) >0.005 and core set of about 12 549 273 SNPs and 9 lakh indels with MAF >0.005. Around 73.7% of the SNPs were present in the intergenic regions and around 4% of the SNPs were present in the coding region. All these outcomes from this study can play an important role in the improvement of the Vitis cultivars (Liang et al. 2019).