Читать книгу X-Ray Fluorescence in Biological Sciences - Группа авторов - Страница 88

EDXRF being a comparison method of elemental analysis requires the construction of a calibration curve to validate results. This curve is obtained by irradiating a commercially available standard reference material (SRM) (prepared in the same manner as the real sample) of a similar matrix. The basic concept operative here is that both the real sample and SRM should produce identical sensitivity, thus nullifying the matrix effect. In this study, commercially available standard reference material Spinach (NIST 1570a) was used to construct the calibration curve [15], taking into account that plants and blood have similar biological matrices. The constructed calibration curve has been validated by analyzing CRM (certified reference material) Orchard Leaf (NIST1571) and the obtained values were found to be in good agreement with the certified values. The relative error and coefficient of variation in percentage were in the acceptable range (<10%) as shown in Table 6.2.