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The factors affecting trehalose lipids production can be divided in two major groups: (i) nutritional and (ii) environmental.

i) Nutritional factors

The carbon source can influence the biosurfactant synthesis either by induction or repression, it has considerable bearing on the produced type of biosurfactants. Furthermore nitrogen can play an important role in the regulation of biosurfactant synthesis (Cameotra and Makkar 1998; Ribeiro et al. 2013; Santos et al. 2016).