Читать книгу Nitric Oxide in Plants - Группа авторов - Страница 23

As plants can survive and grow without nitrite and nitrate, they should have nitrite-independent, oxidative mechanisms for NO synthesis. Indeed, analogous to animal nitric oxide synthase (NOS) (EC 1.14.13.39), the plant enzyme is not dependent on nitrite and is involved in the deamination of L-arginine into L-citrulline and NO via NADPH and O₂ and necessitating Ca²⁺/calmodulin (Rőszer 2012). Enzymatic oxidation has been witnessed in the peroxisomes (leaf), chloroplasts of the tracheophytes, and in green algae. In chloroplasts, the oxidation of L-arginine to NO requires NADPH and is independent of Ca²⁺availability. While in the leaf peroxisomes, the conversion of L-arginine/L-citrulline requires Ca²⁺/calmodulin, flavin mononucleotide (FMN), FAD, and NADPH (Mur et al. 2013).