Читать книгу Principles of Virology - Jane Flint, S. Jane Flint - Страница 158

Haploid cell lines have been used to identify genes required for viral reproduction. These cells, which have only one copy of each chromosome, are infected with retroviruses under conditions where one integration event occurs per cell. The disruption of individual genes that are essential for viral replication can be identified by the isolation of cells resistant to infection (Fig. 3.13). Surviving cells are expanded and the site of proviral integration is determined by PCR and high-throughput sequencing. This approach has been used to identify receptors for viruses, including ebolavirus, Lassa virus, and hantavirus, and genes required for receptor modification and endosomal trafficking.

While powerful, a drawback of this approach is that only a few haploid cell lines are available, and not all viruses can infect these cells.