Читать книгу Principles of Virology - Jane Flint, S. Jane Flint - Страница 164

Fiers W, Contreras R, Duerinck F, Haegeman G, Iserentant D, Merregaert J, Min Jou W, Molemans F, Raeymaekers A, Van den Berghe A, Volckaert G, Ysebaert M. 1976. Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene. Nature 260:500–507.

The first complete genome sequence of any kind.

Taniguchi T, Palmieri M, Weissmann C. 1978. QB DNA-containing hybrid plasmids giving rise to QB phage formation in the bacterial host. Nature 274:223–228.

The first infectious virus from a cloned DNA copy of a viral genome.

Crotty S, Cameron CE, Andino R. 2001. RNA virus error catastrophe: direct molecular test by using ribavirin. Proc Natl Acad Sci U S A 98:6895– 6900.

Only a two-fold increase in poliovirus genome mutations is needed to push the population over the error threshold.

Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. 2001. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411:494–498.

The first report that synthetic siRNAs can silence gene expression in mammalian cells.

Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. 2012. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337:816–821.

The Cas9 endonuclease is engineered into a two-component system by fusing the two RNA molecules into a single-guide RNA that, when combined with Cas9, cuts the DNA target specified by the guide RNA.

Wolf YI, Kazlauskas D, Iranzo J, Lucía-Sanz A, Kuhn JH, Krupovic M, Dolja VV, Koonin EV. 2018. Origins and evolution of the global RNA virome. mBio 9:e02329–18.

Analysis of RdRp sequences illuminates RNA virus evolution.