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5.5 Granulocytes

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Granulocytes for transfusion are prepared by cytapheresis (see Chapter 6). Some investigators have prepared granulocytes from fresh WB by sedimentation with hydroxyethyl starch to obtain doses of 0.25 × 109. This is below the 1–3 × 109 desired for transfusion even to a neonate [92]. The possibility of obtaining granulocytes from units of WB is usually raised in a crisis; the blood bank often does not have procedures to prepare the cells, and it is not possible logistically to test the blood for transmissible disease. Thus, preparation of granulocytes from units of fresh WB is not recommended.

Transfusion Medicine

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