Читать книгу Basic Virology - Martinez J. Hewlett - Страница 52

Infection of rabbit eyes with HSV leads to localized infection and recovery. The rabbits maintain virus in their trigeminal ganglia, and viral DNA, virus, or both can be recovered using methods described for the murine model. Unlike mice, rabbits spontaneously reactivate HSV, and virus occasionally can be recovered from the rabbit's tear film. Further, this reactivation can be induced by iontophoresis of epinephrine with high frequency. Rabbits, because HSV can reactivate in them, are vital to the design of experiments to investigate induced reactivation, although they are more expensive to purchase and keep than mice.

Figure 3.6 Analysis of the establishment and maintenance of latent HSV infections in mice. A number of mice are inoculated in the footpad, and following the symptoms of primary disease, which include foot swelling and minor hindquarter paralysis, many mice recover. Those that do not recover have infectious virus in their CNS. The mice that recover are latently infected and no infectious virus can be detected, even with high‐sensitivity measurements of nervous and other tissue. HSV genomes, but not infectious virus, can be detected in nuclei of sensory nerve dorsal root ganglia. When these ganglia are cultured with other cells that serve both as an indicator of virus replication and as a feeder layer for the neurons (i.e., explanted), a significant number demonstrate evidence of virus infection and infectious virus can be recovered, as shown on the inset graph (two separate experiments are shown, with essentially the same results).