Читать книгу Essential Endocrinology and Diabetes - Richard I. G. Holt - Страница 86

 Avoid use of ‘drip arm’ to avoid dilution or contamination

 Avoid prolonged use of tourniquetVenous stasis can affect measurement of calcium and protein.

 Ensure efficient techniqueStress of venepuncture can increase prolactin and other stress hormonesAvoid haemolysis

 Ensure correct tube typeLithium heparin: most hormonesMay interfere with ACTH assaysFluoride oxalate: glucoseMay interfere with IGFBP‐3 assaysEDTATests requiring DNA isolationImproves stability of peptides but can interfere with some enzyme testsAvoid risk of contamination by ensuring correct ‘order of draw’plain → citrate → Li heparin → EDTA → fluoride.

 Take an adequate volume of sample

 Ensure samples are properly labelled