Читать книгу Fundamentals of Analytical Toxicology - Robin Whelpton - Страница 44

To collect erythrocytes, heparinized blood should be centrifuged (2000 g, 10 min), the plasma, buffy coat, and top 10 % of erythrocytes (mainly reticulocytes) removed, and the remaining erythrocytes carefully washed with isotonic phosphate-buffered saline (PBS), pH 7.4, to remove trapped plasma. The cells may be used directly or stored at –20 °C, either to cause haemolysis, or for storage. Platelets are usually isolated by the slow centrifugation (e.g. 300 g, 15 min) of anticoagulated whole blood to yield platelet-rich plasma, which is recentrifuged (2000 g, 10 min) to harvest the platelets. Other white blood cells are most commonly obtained either by centrifugation through media of appropriate density (according to manufacturers' instructions), or isolated by solid-phase antibody techniques.