Читать книгу Fundamentals of Analytical Toxicology - Robin Whelpton - Страница 56

In order to maximize the reliability of measurements performed in post-mortem blood, it is recommended that (i) the interval between death and the post-mortem examination is minimized, (ii) the body/samples are stored at 2–8 °C before the examination/after collection, (iii) that blood is collected from two distinct peripheral site(s), preferably the femoral vein(s), after tying off the vein proximally to the site of sampling (Lemaire et al., 2016), and (iv) a preservative (approximately 2 % w/v, fluoride) is added to a portion of the blood sample/the sample from one vein. The exact site of blood sampling should be recorded, as should the time of sampling and (approximate) time of death if known.

If sufficient sample is obtained, this should be divided between unpreserved and preserved (fluoride) tubes, otherwise the entire sample should be preserved unless there is a possibility of poisoning with fluoride or compounds giving rise to fluoride in vivo such as fluoroacetate. Added fluoride may also enhance decomposition of organophosphorus compounds (Moriya et al., 1999). If only heart or cavity blood is available this should be clearly stated.

Post-mortem blood (ca. 20 mL) for qualitative analysis only should be taken from the heart (preferably right atrium), inferior vena cava, or another convenient large vessel. The precise sampling site must be recorded on the sample tube. The blood should be free-flowing.