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BOX 1.1 Structural Features of Bacterial Genomes

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It is widely appreciated that the chromosomes are the information storehouse for an organism. What is less appreciated is that the chromosome as a structure has evolved sequence motifs that allow it to be efficiently replicated, repaired, and segregated into daughter cells. The distribution and orientation of these motifs are discussed here; the molecular biology of the systems that recognize these sequences is explained in greater detail in the text. The placement of these sequence motifs in the context of the chromosome is important for their function, as is the orientation of many of these sequences. Many of the motifs are oriented in one direction, which follows the direction of the DNA replication fork. DNA replication in E. coli and B. subtilis (and all bacteria studied to date) is initiated within a single oriC region and continues bidirectionally to a position on the chromosome equidistant from the origin (indicated by the long arrow-headed line in the figure). The dif site where the resolution of dimer chromosomes occurs is found near to where DNA replication normally terminates.

Certain DNA sequence motifs that guide DNA replication and DNA repair are polar in that they are not symmetrical and need to be in a specific 5′-to-3′ direction to carry out their functions. In other words, these sequences must be found in a certain orientation in the chromosome and will not work if they are flipped around. In E. coli and B. subtilis, DNA replication forks are actively terminated at specific sites called ter sites. The ter sites act as a trap for DNA replication forks, and these sites encompass a large portion of the chromosome, allowing DNA replication forks to pass when approaching from one direction but not the other. Multiple ter sites (10 in E. coli and 9 in B. subtilis) are found in the genome, and the redundancy of these sites may be important for catching replication events that get through the initial ter sites (indicated with a dashed line) or to stop replication forks that are initiated for DNA repair by recombination (see chapter 9). For unknown reasons, the central ter sites in B. subtilis are very close together, while the central sites in E. coli are separated by hundreds of thousands of base pairs. Along the path of DNA replication are sequence motifs involved in guiding the DNA translocase proteins involved in chromosome segregation: FtsK, found in E. coli (which recognizes motifs called KOPS), and SpoIIIE, found in B. subtilis (called SRS motifs). The StpA DNA translocase from B. subtilis may also recognize the SRS sites. Chromosomes also have polar DNA sequences that guide the recombination machinery (see chapter 9). DNA recombination is extremely important in bacteria as a way to repair DNA double-strand breaks that occur during DNA replication. Repair of these breaks occurs when recombination reestablishes a DNA replication fork using one broken end and the sister chromosome. Reestablishment of DNA replication involves an efficient processing event that utilizes polar sites called chi sites. The RecBCD complex in E. coli or the AddAB complex in B. subtilis carries out this processing activity using information found in the chi sites. chi sites are species specific and are common in genomes in one orientation from the origin to the terminus region on the leading strand (found about 1 every 5 kb in the E. coli chromosome).


Other polar sequence biases in the chromosome include an overrepresentation of the 5′-CTG-3′ sequence that primes lagging-strand DNA synthesis (not shown). Interestingly, the most common triplet codon is the CUG (5′-CTG-3′ in DNA) that codes for leucine, comprising almost 5% of all codons in E. coli. The 5′-CTG-3′ sequence is found in the chi sequence and all of the most frequent 8-bp sequences in the chromosome. It would be difficult to argue which came first, the use of this sequence by the primase or its frequency of use as a codon. Another type of sequence bias, but one that is not polar in nature, is a general sequence bias called the G/C skew, where G and C are overrepresented in the leading strand. The trend toward A and T in the lagging strand is believed not to have an adaptive value but to be a result of the way in which repair differs on the two strands.

There are other DNA sequences that are not polar but that show biases for regions of the chromosome. Around the origin of B. subtilis, an area recognized by the Spo0J protein for segregation of the origin region to daughter cells, the parS sequence, is found 8 times (the orange rectangular boxes in the figure). Also around the origin of B. subtilis, there is an enrichment of ram sites (short yellow dashes), a sequence recognized by the RacA protein for maintaining segregation during sporulation. Binding sites for the nucleoid occlusion proteins which prevent septum formation until division is nearly complete reside across the chromosome but are absent from the terminus region. SlmA-binding sites (SBS) in E. coli and Noc-binding sites (NBS) in B. subtilis (long brown dashes) are recognized by SlmA and Noc, respectively. The GATC sites (not shown), which are important for regulating the initiation of DNA replication at oriC in E. coli, also show enrichment in the oriC region, with a spacing that is important for SeqA binding. The organization of the large domain comprising the terminus region of the chromosome appears to be important in E. coli, where the MatP protein recognizes matS sites (red dashes in the E. coli diagram) found across this region.

Snyder and Champness Molecular Genetics of Bacteria

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