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6.7.2 Application of pollen

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Commonly, pollen is applied directly to the stigma by using a fine brush or dusting off the pollen onto the stigma directly from the flower of the pollen source (e.g. the staminal column may be used as brush). Sometimes, objects such as cotton bulbs or toothpicks are used to deposit pollen on the stigma. In some flowers, pollen deposition is made without direct contact with the stigma. Instead, pollen may be injected or dusted into a sack covering the emasculated inflorescence and agitated to distribute the pollen over the inflorescence. A key precaution against contamination during pollination is for the operators to disinfect their hands and tools between pollinations, when different varieties are involved. It is critical to tag the pollinated flower for identification at the time of harvesting.

Principles of Plant Genetics and Breeding

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