Читать книгу Phytomicrobiome Interactions and Sustainable Agriculture - Группа авторов - Страница 35

Microorganisms from the endosphere penetrate and invade into the root internal tissues. A vast array of plants' endosphere is colonized by fungi (Vandenkoornhuyse et al. 2002; Smith and Read 2008), bacteria (Reinhold‐Hurek and Hurek 2011) and, some time by Archaea (Sun et al. 2008). The molecular tools to increase our comprehension about the endophytic lifestyle within the host plant are poorly understood (Reinhold‐Hurek and Hurek 2011). Metagenomic studies might be helpful to lengthen advancement in this enthralling research area. Furthermore, the metagenomics approach can also be helpful to elucidate the potential of uncultivable endophytic microbial communities, thus revealing the information beyond the genomic information of an individual in the endosphere microbiome (Dinsdale et al. 2008). Moreover, metagenomic studies mainly depend upon the quality of metagenomic DNA extracted from environmental samples. Also, the prevalence of genomic DNA from the plant biomass limits the application of bacterial transcriptome sequencing or metagenome (Utturkar et al. 2016). Here, significant biases in the DNA extraction methodology from endophytic communities may be introduced (Kgomotso et al. 2015). In this context, some workers also observed that the recovery of a diverse class of endophytic communities associated with plant tissues could be enabled by using broad‐spectrum SDS‐based DNA extraction protocol (Maropola et al. 2015). Separation of the host's genome from the endophytic microbiome's metagenome is a challenging assignment. Due to this, it is rare to achieve full sequencing of an endophytic microbiome by complex procedures like extensive deep genome sequencing (Campisano et al. 2014). However, reports are available which enlighten the endophytic microbiome up to some extents using metagenomics (Tian et al. 2015; Vandenkoornhuyse et al. 2015; Sengupta et al. 2017). Utilizing metagenomic analysis, Sessitsch et al. (2012) reported the functional characteristics of an endophyte community that colonizes rice roots. Similarly, metagenomics workflow analysis of endophytic bacteria from oil palm fruits has been described by Tanjung and coworkers (Tanjung et al. 2017).