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3.6.2 Quantitative Insights into Microbial Ecology (QIIME)

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QIIME (http://qiime.org) is also an open‐source bioinformatics tool that is designed to generate quality graphics and statistics from raw data generated from Illumina or other platforms (Caporaso et al. 2010). The OTUs (operational taxonomic units) picking is the main output from QIIME pipeline, which illustrates the abundance of sequence variant in quarry samples. Other features such taxonomic assignment, phylogenetic reconstruction, and diversity analyses and visualizations provide additional strength to QIIME. Recently, an advance variant of QIIME, QIIME2 (https://qiime2.org) has been launched (Bolyen et al. 2019). Its q‐2‐micom plug‐in allows users to build and simulate metagenome‐scale metabolic community models and they can also predict metabolic fluxes, which are undergoing in microbial consortium.

Phytomicrobiome Interactions and Sustainable Agriculture

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