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2.8 Conclusion

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Although the metagenomic approach for detecting microbes that produce novel biosurfactants is still at a growing stage, there is an ample opportunity to use these molecular tools in the future. As of now, these techniques have been used in the collection of environmental DNA samples for research purposes. In the above sections, we discussed metagenomic approaches to microorganism screening, which produce biosurfactants with add‐on techniques to overcome barriers faced by conventional metagenomics. Emphasis has been placed on the synergy of techniques and methodologies with a functional or sequence‐based approach for more favorable outcomes. We present how DNA‐SIP coupled with metagenomic strategies can help identify specific microbes of interest. The technique could be exploited in screening microbes or genes producing novel biosurfactants from oil‐contaminated soil. In addition to the above, we emphasize the characteristics and limitations of biosurfactant screening methods. The challenges and opportunities for functional metagenomics have been presented in detail.

Biosurfactants for a Sustainable Future

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