Читать книгу pH of the Skin: Issues and Challenges - Группа авторов - Страница 73

After measuring the baseline pH of untreated skin-mimic collagen film surface, emollient was uniformly applied to 2 new pre-weighed 80 cm² pieces of skin-mimic film. Based on measured weight gain, the two emollient application rates were 0.26 and 0.17 mg/cm². Collagen skin-mimic film samples were large enough to make multiple pH measurements in different locations (Coffi brand, 25 μm; Naturin Viscofan, Weinheim, Germany). The Skincheck pH meter was used to measure the non-emollient side of the skin-mimic film initially and then at time intervals up to 24 h with the emollient skin-mimic film pieces stored at 37°C. This essentially simulates the recording of a pH measurement 25 μm below the surface of skin that has topsheet emollient applied to the surface of the skin. A non-emollient collagen skin-mimic film control was also measured at each time interval.