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Mycoplasma is not a significant problem in AFC culture, due mainly to better quality control by the serum manufacturers but also to the awareness of cell culturists that AFC cultures should not share incubator space with established cell lines. A shared water bath used for heating media and trypsin can be a source of mycoplasma contamination because permanent cell lines, frequently shipped from laboratory to laboratory, remain the prevailing source of mycoplasma contamination. As additional protection, many laboratories heat‐inactivate their sera before use. Commercial test kits are available for the detection of mycoplasma infections in cell cultures.⁶⁷¹