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Incubator failure is not a trivial cause of culture loss. The main threats are breakdown of the gas supply or equipment. AF cells cannot tolerate a pH close to or higher than 8 for more than 6–8 hours. On the other hand, a pH of less than 7.0 (for example, due to excess CO₂ in the incubator) causes cells to stop dividing. A second danger is overheating of incubators due to mechanical failure or human error. Connection of incubators to emergency power sources is important. Temperature‐ and gas‐sensitive alarm systems are advisable.