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Once the semen sample has been collected the vial can be easily detached from the semen collection cone. To avoid spillage it works best to pull the cone sideways and slightly downward away from the vial, which has the effect of stretching and shearing the cone off on the edges of the vial. Always keep the semen vial protected from the cold prior to evaluation. A simple and effective method is to hold the vial in a warm hand with the fingers completely encircling it. If evaluation is not going to occur right away the sample should be transferred to a 37 °C water bath. The chest pocket of a close‐fitting pair of coveralls has also worked well for short‐term storage, especially if the hands are needed for other tasks.

Although not always recorded, the skilled examiner should know how to assess each of the following when examining a semen sample:

 Color and consistency

 Volume

 Concentration

 Gross motility

 Individual motility

 Sperm morphology

 Percentage staining alive