Читать книгу Bovine Reproduction - Группа авторов - Страница 140
Gross Motility
ОглавлениеGross motility or gross wave motion is influenced by the percentage of progressively motile sperm, the sperm concentration, and the vigor or rate of speed of the motile sperm. Determining gross motility is a fast and simple method requiring just a drop of semen on a microscope slide which is then viewed using bright field microscopy at 40–125× magnification [2]. Vigorous swirls and eddies are indicative of a concentrated semen with a very high proportion of vigorous motile sperm. A depression affecting one or more of the three factors will, in turn, have a negative effect on gross motility. For example, sperm motility may be very high in a poorly concentrated semen sample yet the gross motility appears poor. Another common occurrence is when a highly concentrated sample becomes chilled, negatively affecting sperm vigor. As a result, gross motility should be interpreted carefully, leaving individual motility as a more reliable method of assessing sperm motility.