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Individual Motility

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Individual motility or more precisely percentage of progressively motile sperm is determined by placing a 2‐ to 4‐mm drop of semen on a clean microscope slide over which a cover slip is dropped in place. The seminal fluid should spread just to the edge of the cover slip to form a thin layer in which the sperm can be viewed within the same focal plane [2]. Too much semen and the cover slip will float, making visualization of individual sperm more difficult, and very concentrated samples may be impossible to evaluate without dilution. Although readily available, the low pH of physiologic saline can diminish sperm motility. Warmed sodium citrate solution [17], commercial semen extender, or even fresh sperm‐free seminal fluid are better choices to use as a diluent [2]. Individual motility is recorded as a percentage, with minimum acceptable proportions of 30% [1] and 60% [2] being used by the respective SFT and Western Canadian Association of Bovine Practitioners' guidelines. Several fields should be examined at 400× magnification, observing the proportion moving forward versus those that are immotile, or barely motile, and an average of the percentage motile sperm can be estimated, usually rounded to the nearest multiple of 10. Individual sperm motion is most effectively observed using phase contrast microscopy. Most modern microscopes can be fitted with phase contrast for a modest cost. Sperm motility can be easily affected by heating, chilling, and contamination with urine or other fluids, soaps, etc. A finding of poor motility should be substantiated by sperm morphology and vitality (percentage staining live) before it is considered reflective of fertility. If a finding of poor motility cannot be substantiated then a second sample should be evaluated.

Bovine Reproduction

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