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As shown above, there are diverse microorganisms able to produce BS, but few of them have the kinetic characteristics for large‐scale production since bio‐based product market is appealing [83] for new or better‐producing microorganisms and is a challenge for biotechnology. Microbial screening has been performed on different environments, such as hydrocarbon and oil‐contaminated soil, coastal and offshore [84–86], gas platforms [87], seawater biofilm [88], marine environment [89], mangrove sediments [90], food materials [91], and Amazon rainforest [92]. Many of the isolates are bacteria related to Pseudomona and Bacillus genera. In the case of yeasts, Candida, Starmerella (ex Candida), and Pseudozyma are the most used genera; but more microorganisms are emerging nowadays as BS producers.

Accurate methodologies for isolation and characterization of microorganisms are important to discover specific metabolic capabilities. By traditional methods, microorganisms are isolated from environmental samples and tested for BS production by qualitatively, e.g. CTAB agar assay or hemolysis test as a primary approach. A different strategy limits the screening to oil or hydrocarbon‐contaminated sites, resulting in an increment of isolates related to BS production [93]. In both cases, it is necessary to employ quantitative or semi‐quantitative methods for BS evaluation: drop collapse test, emulsion index, TLC and superficial tension, or techniques such as HPLC/UPLC, LC‐MS, or MS. Nevertheless, this methodology is only for cultivable microorganisms, so there is an undetermined number of potential producers in different environments. At this moment, the metagenomic approach is not a common practice for the screening of BS producers [94].