Читать книгу Genotyping by Sequencing for Crop Improvement - Группа авторов - Страница 61

Arabidopsis thaliana belongs to the family Brassicaceae. It has 125–150 Mb diploid genome having around 30,000 protein‐coding genes distributed over five chromosomes. Weigel and Mott (2009) initiated a project to report whole‐genome sequence variation in 1001 accession. To sequence species‐wide genome of the Arabidopsis, they proposed an approach with two different aspects. In the first aspect, by using the technologies such as Roche’s 454 platform they generated a small number of sequences that approach the quality of A. thaliana’s original Col‐o (Columbia) reference. For a large number of sequence, a relatively less expensive technology for example Applied Biosystem’s SOLiD or Illumina’s Genome Analyzer was used. Local haplotype similarity was exploited by using the information from the reference genome to draw a complete genome sequence. In a second aspect of this approach, the sampling was done for ten individuals from ten populations and geographical regions all around the Eurasia and at least one accession from North Africa (10 × 10 × 10 + 1). The aim of this 1K Arabidopsis genome project was to sequence a generalized genome that can encompass every Arabidopsis accession and every genome of the A. thaliana can be completely aligned against it (Weigel and Mott 2009).