Читать книгу Genotyping by Sequencing for Crop Improvement - Группа авторов - Страница 43

Among the conventional molecular markers, RFLP is based on the detection of mutation at the restriction site which is usually SNP. Except for RFLP, which relies on restriction digestion followed by detection of digested DNA via southern blotting, other majority of the SNP genotyping methods require amplification of the SNP containing genomic region prior to polymerase chain reaction (PCR). This preamplification step is inevitable from most of the genotyping techniques, despite the fact that the PCR process is relatively expensive.