Читать книгу Genotyping by Sequencing for Crop Improvement - Группа авторов - Страница 40

First, a diverse set of genotypes are selected based on phenotype for the creation of SNP panel with the concept that as these genotypes are hypervariable in several phenotypes, the same should reflect from their sequences. The reference genome sequence is used for reference‐based assembly of the reads generated from sequencing of these genotypes (S1–S10 in Figure 2.1). A set of hypervariable loci are filtered through some statistical analysis. Once discovered, these can be genotyped in any other accession, which is known as SNP genotyping, as genotyping is the process of knowing the allelic status of an organism at a particular locus. The process till the identification of hypervariable loci is known as SNP discovery. The discovery of SNPs in crops prompted the development of the simple and low‐cost genotyping platforms. A flowchart related to different SNP genotyping technologies has been provided (Figure 2.2).

Figure 2.1 A pipeline for SNP discovery (S1–S10 are different diverse accession of the same species to which the reference belongs).