Читать книгу Genotyping by Sequencing for Crop Improvement - Группа авторов - Страница 26

The improvement of Sanger sequencing technology in the 1990s combined with the beginning of EST and genome sequencing projects in model plants led to the spurt in the identification of variation at the single‐base resolution (Wang et al. 1998). From 2005 onward, the emergence of NGS platforms such as Roche 454, Illumina HiSeq2500, ABI 5500xl SOLiD, Ion Torrent, PacBio RS, Oxford Nanopore, and advances in bioinformatics tools simplified the process of identification of genome‐wide SNPs and changed the face of molecular marker technology. NGS‐based genotyping platforms such as genotyping‐by‐sequencing (GBS), whole‐genome resequencing (WGR), and high‐density SNP arrays helped to type thousands of SNPs in a single reaction in hundreds of individuals.