Читать книгу Genotyping by Sequencing for Crop Improvement - Группа авторов - Страница 39

Accurate detection of SNP is the first and foremost step for its mainstream application in breeding experiments. Besides the sequencing techniques used, reads length, coverage, and robust assembly approach are the control points for sequencing errors. Sequencing technologies with long reads length (in kb’s) like PacBio single‐molecule real‐time (SMRT) sequencing (Eid et al. 2009), Illumina TruSeq synthetic long‐read technology (McCoy et al. 2014), and Oxford nanopore sequencing (Branton et al. 2010) are available to develop the complete and correct reference genome assembly of any organism, which sort out many of the problems in SNP discovery. Not only in genome sequencing, these long‐read methodologies have been successfully deployed for SNP mining and provide a more precise understanding of the complexity of isoforms for organisms lacking a reference (Piriyapongsa et al. 2018). Once a reference genome is available, the application of SNPs in its breeding program becomes comparatively easier. A concise layout for SNP‐based breeding experiment is provided (Figure 2.1).