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X-Ray Fluorescence in Biological Sciences
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Страница 1
Table of Contents
List of Tables
List of Illustrations
Guide
Pages
X‐Ray Fluorescence in Biological Sciences Principles, Instrumentation, and Applications
Страница 8
List of Contributors
Preface
Страница 11
1 X‐Ray Fluorescence and Comparison with Other Analytical Methods (AAS, ICP‐AES, LA‐ICP‐MS, IC, LIBS, SEM‐EDS, and XRD)
1.1 Introduction
1.2 Analytical Capabilities of XRF and Micro‐XRF
1.2.1 Micro‐XRF
1.3 Comparison with Other Analytical Methods 1.3.1 Overview
1.3.2 Inductively Coupled Plasma (ICP) Analysis
1.3.2.1 Inductively Coupled Plasma Mass Spectrometry (ICP‐MS)
1.3.3 Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP‐AES)
1.3.4 Ion Chromatography (IC)
1.3.5 Laser‐Induced Breakdown Spectroscopy (LIBS)
1.3.6 Proton‐Induced X‐Ray Emission (PIXE)
1.3.7 Scanning Electron Microscopy–Energy Dispersive X–Ray Spectroscopy (SEM‐EDS)
1.3.7.1 Differences in XRF and SEM‐EDS (Sample Handling, Experimental Conditions, Sample Stress, and Excitation Sources)
1.3.7.2 Combination of SEM‐EDS and μ‐XRF
1.4 Comparison of XRF and XRD
1.5 Comparison of XRF and Raman Spectroscopy
1.6 Conclusion and Prospects
References
2 X‐Ray Fluorescence for Multi‐elemental Analysis of Vegetation Samples
2.1 Introduction
2.2 Features and Analytical Capabilities of XRF Configurations used in Vegetation Sample Analysis
2.3 General Sample Treatment Procedures used for Vegetation Sample Analysis using XRF Techniques
2.4 Applications of XRF in the Field of Vegetation Samples Analysis 2.4.1 Environmental Studies
2.4.2 Nutritional and Agronomic Studies
2.5 Concluding Remarks and Future Perspectives
References
3 X‐Ray Fluorescence Studies of Tea and Coffee
3.1 Introduction
3.2 The Equipment Used
3.3 Preparation of Samples for Analysis
3.4 Examples of Practical Applications of XRF for Tea Research
3.5 Examples of Practical Applications of XRF for Coffee Research
3.6 Determination of the Elemental Composition of Krasnodar Tea Samples by TXRF and WDXRF
3.6.1 Instrumentation
3.6.2 Suspension Preparation
3.6.3 Infusion Preparation
3.6.4 Acid Digestion
3.6.5 Preparation of Samples for WDXRF
3.6.6 Results and Discussion
3.7 Interelement Effects and Procedures of their Accounting
3.8 Conclusion
References
4 Total Reflection X‐Ray Fluorescence and it’s Suitability for Biological Samples
4.1 Introduction
4.2 Advantages and Limitations of conventional XRF for Elemental Determinations in Biological Systems
4.3 Factors Limiting the Application of XRF for Biological Sample Analysis
4.4 Modifying XRF to Make it Suitable for Elemental Determinations at Trace Levels: Total Reflection X‐Ray Fluorescence (TXRF) Spectrometry
4.4.1 Principles of TXRF
4.4.2 Theoretical Considerations
4.4.3 TXRF Instrumentation for Trace Element Determination
4.4.4 Sample Preparation for TXRF Analysis
4.5 Suitability of TXRF for Elemental Analysis in Biological Samples
References
Note
5 Micro X‐Ray Fluorescence and X‐Ray Absorption near Edge Structure Analysis of Heavy Metals in Micro‐organism
5.1 Introduction
5.2 Effects of Heavy Metals on Microbial Growth
5.3 Application of μ‐XRF and XAS in Understanding the Cycling of Elements Driven by Micro‐organism
5.4 Application of μ‐XRF and XAS in Understanding the Transformation of Elements Driven by Micro‐organisms
5.5 Application of μ‐XRF and XAS in Understanding the Mechanism of Using Micro‐organisms in Bioremediation
5.6 The Advantage of Using μ‐XRF and XAS to Explore the Interaction Mechanism Between Micro‐organisms and Heavy Metals
References
6 Use of Energy Dispersive X‐Ray Fluorescence for Clinical Diagnosis
6.1 Introduction
6.2 Determination of Arsenic Concentration in Human Scalp Hair for the Diagnosis of Arsenicosis Disease 6.2.1 Background
6.2.2 Role of EDXRF
6.2.3 Collection and Preparation of Hair Sample
6.2.4 Sample Preparation
6.2.5 Sample Analysis
6.2.6 Accuracy and Precision of the Method 6.2.6.1 Construction of Calibration Curve
6.2.6.2 Measured Condition
6.3 Determination of Lead Concentrations in Human Whole Blood Using EDXRF Technique with Special Emphasis on Evaluating Association of Blood Lead Levels with Autism Spectrum Disorders (ASD) 6.3.1 Background
6.3.2 Role of EDXRF in Diagnosis of Blood Lead Level
6.3.3 Collection of Blood Sample and Preparation
6.3.4 Preparation of Pellets from Powdered Sample
6.3.5 Sample Irradiation
6.3.6 Precision and Accuracy of the Result
6.4 Conclusion
References
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