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C57BL/6J male mice were orally dosed with carbon tetrachloride (CCl₄), because there are numerous reports that CCl₄ induces hepatotoxicity in many experimental animals (Chopra et al. 1972). Whole blood and liver samples were collected twenty-four hours after the administration of CCl₄ (0 mg/kg (vehicle control: corn oil), 7 mg/kg and 70 mg/kg) for the following experiments.

Hematoxylin and eosin (H&E) staining showed CCl₄ (70 mg/kg)-induced histopathological changes in the liver, with significant degeneration and necrosis of hepatocytes in the centrilobular region and perivenular inflammatory infiltrates twenty-four hours after administration, while histological tissue sections of mice in the vehicle control group and CCl₄ (7 mg/kg) showed a normal histological morphology of liver tissue samples (Figure 3.9).

Figure 3.9 Representative H&E micrographs of liver tissues collected from mice treated with oral administration of corn oil (control) (a), 7 mg/kg CCl₄ (b), and 70 mg/kg CCl₄ (c). The control section shows the normal histological structure of the central vein (cv) and surrounding hepatocytes (a). Twenty-four hours after 7 mg/kg CCl₄ treatment, there were no histopathological changes by comparison with the control section (b). Twenty-four hours after 70 mg/kg CCl₄ treatment, the hepatocytes around the central veins (cv) were vacuolized and necrotic (c).