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Yan and coworkers incorporated single‐stranded DNAs to detect target RNA molecules at the single‐molecule level on the surface of DNA origami (Figure 1.7a–c) [48]. Even though samples containing large amounts of cell‐derived RNAs were used, the binding of the target RNA could only be visualized by AFM, and nonspecific binding was not observed. Because DNA origami tiles carry different types of complementary DNAs and corresponding hairpin DNA markers, binding of target RNAs could be identified from the specific hairpin markers on the DNA origami even though the different origami tiles were mixed. In this study, the detection limit of RNA molecules was approximately 1000 molecules, meaning that the target RNA could be directly detected from a single cell without using polymerase chain reaction (PCR) amplification.