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Gothelf and coworkers detected selective bond cleavage and bond formation reactions on a DNA origami surface. Target molecules having specific reactivity were introduced at specific positions on DNA origami. Reductive cleavage of disulfide bonds and oxidative cleavage of an olefin by singlet oxygen were carried out on the DNA origami surface, and the reactions proceeded quantitatively at the single‐molecule level [49]. In addition, amide bond formation and click reactions were performed with 80–90% yield, and three successive reactions were also performed (Figure 1.7d,e). These chemical reactions were monitored by the cleavage of biotin‐attached chemical linkers and bond formation with biotin‐tethered functional groups, which can be labeled with streptavidin for visualization by AFM.