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DNA Origami
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Table of Contents
List of Tables
List of Illustrations
Guide
Pages
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1 DNA Origami Technology: Achievements in the Initial 10 Years
1.1 Introduction
1.1.1 DNA Nanotechnology Before the Emergence of DNA Origami
1.2 Two‐Dimensional DNA Origami
1.3 Programmed Arrangement of Multiple DNA Origami Components
1.4 Three‐Dimensional DNA Origami Structures
1.5 Modification and Functionalization of 2D DNA Origami Structures 1.5.1 Selective Placement of Functional Nanomaterials
1.5.2 Selective Placement of Functional Molecules and Proteins via Ligands
1.5.3 Distance‐Controlled Enzyme Reactions and Photoreactions
1.6 Single‐Molecule Detection and Sensing using DNA Origami Structures 1.6.1 Single‐Molecule RNA Detection
1.6.2 Single‐Molecule Detection of Chemical Reactions
1.6.3 Single‐Molecule Detection using Mechanical DNA Origami
1.6.4 Single‐Molecule Sensing using Mechanical DNA Origami
1.7 Application to Single Biomolecule AFM Imaging 1.7.1 High‐Speed AFM‐Based Observation of Biomolecules
1.7.2 Visualization of DNA Structural Changes in the DNA Nanospace
1.7.3 Visualization of the Reaction Events of Enzymes and Proteins in the DNA Nanospace
1.8 Single‐Molecule Fluorescence Studies
1.8.1 Nanoscopic Ruler for Single‐Molecule Imaging
1.8.2 Kinetics of Binding and Unbinding Events and DNA‐PAINT
1.8.3 DNA Barcode Imaged by DNA‐PAINT
1.9 DNA Molecular Machines
1.9.1 DNA Assembly Line Constructed on the DNA Origami
1.9.2 DNA Spider System Constructed on the DNA Origami
1.9.3 DNA Motor System Constructed on the DNA Origami
1.10 Selective Incorporation of Nanomaterials and the Applications
1.10.1 DNA Origami Plasmonic Structure with Chirality
1.10.2 Surface‐Enhanced Fluorescence by Gold Nanoparticles and DNA Origami Structure
1.10.3 Placement of DNA Origami onto a Fabricated Solid Surface
1.11 Dynamic DNA Origami Structures Responsive to External Stimuli 1.11.1 DNA Origami Structures Responsive to External Stimuli
1.11.2 Stimuli‐Responsive DNA Origami Plasmonic Structures
1.11.3 Photo‐Controlled DNA Origami Plasmonic Structures
1.12 Conjugation of DNA Origami to Lipid 1.12.1 DNA Origami Channel with Gating
1.12.2 DNA Origami Templated Synthesis of Liposomes
1.13 DNA Origami for Biological Applications
1.13.1 Introduction of DNA Origami into Cells and Functional Expression
1.13.2 Drug Release Using the Properties Characteristic for DNA Origami
1.13.3 DNA Origami Structures Coated with Lipids and Polymers
1.13.4 Nanorobot with Dynamic Mechanism
1.13.5 Nanorobot Targeting Tumor In Vivo
1.14 Conclusions
References
2 Wireframe DNA Origami and Its Application as Tools for Molecular Force Generation
2.1 Introduction
2.2 Pre‐Origami Wireframe DNA Nanostructures
2.3 Hierarchical DNA Origami Wireframe
2.4 Entire DNA Origami Design
2.5 DNA Origami Wireframe as Tools for Molecular Force Application 2.5.1 Introduction
2.5.2 Results and Discussion
2.6 Conclusions
2.6.1 Materials and Methods
References
3 Capturing Structural Switching and Self‐Assembly Events Using High‐Speed Atomic Force Microscopy
3.1 Introduction
3.2 DNA Origami Nanomachines
3.3 Ion‐Responsive Mechanical DNA Origami Devices
3.4 Photoresponsive Devices
3.5 Two‐Dimensional Self‐Assembly Processes
3.6 Sequential Self‐Assembly
3.7 Photostimulated Assembly and Disassembly
3.8 Conclusions and Perspectives
References
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