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1.13.2 Drug Release Using the Properties Characteristic for DNA Origami
ОглавлениеThe twist of the bundled double‐helices can be controlled by changing the pitches which can be adjusted by the number of base‐pairs between crossovers [29]. Högberg and coworkers developed a drug delivery system using a pitch‐controlled 3D origami with Dox (Figure 1.15b) [109]. The 3D origami structure was designed using double helices with different pitches. By designing the DNA origami structures with a pitch of the usual 10.5 base pairs and a looser one of 12 base pairs, the amount of Dox binding to the loose structure increased by 33% compared with the usual type. The amount of incorporated drug can be adjusted, and it can be released slowly over time. The drug was efficiently taken up into cells, confirming that the DNA origami structure is effective for the efficient delivery of Dox. The structure was retained in the cells and apoptosis of cancer cells was effectively induced. This study shows the characteristics of DNA origami that can regulate drug release by designing the structure using different degrees of twist.