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DNA nanostructures provide various scaffolds to place size‐controlled materials. Artificial liposomes are a useful tool for studying membrane structures and for applications such as drug delivery. However, it is difficult to control the size of liposomes in a customized fashion. Lin and coworkers created a method for producing sub‐100 nm‐sized liposomes using a DNA origami template (Figure 1.14c,d) [104]. Ring‐shaped DNA origami structures with different diameters were designed and prepared. Lipid molecules were placed via hybridization of the DNA–lipid conjugate onto the inner surface of the DNA ring. Then, the ring with handles was mixed with additional lipid and detergent and dialyzed to induce liposome formation. Liposomes were released from the ring and showed greater uniformity than those prepared using traditional methods. The liposomes produced by this method have a narrow size distribution, indicating that this method can be used to guide lipid bilayer formation. Furthermore, more complex structures of liposomes such as tubular and circular were created using DNA origami cages [105].