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Seeman and coworkers created an assembly line in which a DNA walker was operated on the DNA origami and captured multiple gold nanoparticles (AuNPs) (Figure 1.11a) [86]. They arranged the three PX‐JX₂ devices and operated a DNA walker that moved on a predesigned track (route). All device movements and DNA walker movements were controlled by specific DNA strands. AuNPs of different sizes were placed on the PX‐JX₂ devices and were transferred to the DNA walker by rotating motion at specific positions. These results show that the DNA walker moved on the track unidirectionally that the delivery of AuNPs was controlled by the PX‐JX₂ devices, and that they were transferred to the DNA walker. After the completion of this process, DNA walkers with three different AuNPs bound were obtained with a yield of 43%. As the PX‐JX₂ device can control the ON–OFF switching of the transfer of AuNPs using specific DNA strands, the product was obtained in high yield (>90%), and the error rate was suppressed (1%). In addition, eight patterns of capture of AuNPs onto the DNA walker were achieved using three PX‐JX₂ devices with the ON–OFF switching operation of these devices.