Читать книгу Core Microbiome - Группа авторов - Страница 50

An analytical database (http://metnetdb.org/mpmr_public/) regarding transcriptome and metabolic data for 14 medicinal plants are available for gene function. In this way, the possible microbial production of glycyrrhetinic acid was recorded. Yamazaki et al. (2013) reported the differential transcriptome analysis along with metabolic profiling, and identified candidate’s genes involved in the biosynthesis pathway of alkaloids and anthraquinones. Transcriptomics of glandular trichomes, of Cannabis sativa flowers, which are the site for cannabinoid biosynthesis, recognized the enzyme involved in cannabinoid biosynthesis, i.e., polyketide synthase, which is responsible for olivetolic acid (and acyl-activating enzyme). Integrated transcriptomics and genetic techniques proposed the presence of a cluster of the gene responsible for alkaloid synthesis in the opium poppy genome (Winzer et al. 2012). Apx1, gene encoding cytosolic ascorbate peroxidase 1 has the characteristics of tolerance to drought and heat stress in Arabidopsis. Three genes of ectoine (compatible osmolyte for salt tolerance) required for biosynthesis were cloned and transferred to tobacco plant Nicotiana tabacum L. bright yellow 2 cv. (Koussevitzky et al. 2007). These three genes are used to enhance tolerance to hyperosmotic shock by the accumulation of ectoine as a result of which normal growth occurs under harsh conditions (Nakayama et al. 2000). Identification and selection of these genes are important processes for introduction into new cultivar to increase the resistance against drought and saline stress. The transcription factor, MsCBF3, could be expressed for the induction of stress tolerance in sensitive genotypes (Purdy et al. 2013). Non-targeted metabolomics analysis results coincide with linking genes function with metabolites, resulting in mutants containing glycosyltransferase and methyltransferase. The genes responsible for the biosynthetic pathway could be characterized and integrated for metabolic profiling, helpful in understanding the stress mechanism in medicinal plants.