Читать книгу Core Microbiome - Группа авторов - Страница 51

Integrated approaches were followed for phytochemical genomics in medicinal plants and their correlation among corresponding elements, such as transcriptome and metabolome, and after which, their successful application in medicinal plants produced bioactive specialized metabolites. For this purpose, next-generation sequencing triggers transcriptome study for an analysis of the co-response of the compound gene that is activated during the stress response. Catharanthus roseus transcriptome co-expression results predicted that specific genes are linked to monoterpenoid indole biosynthesis (Giddings et al. 2011) as a result of converting oxogeranial to iridoid scaffold (Geu-Flores et al. 2012). As part of the chemical expression profiling for quinolizidine alkaloids in Lupinus angustifolius, gene encoding lysine decarboxylase, the first step of the alkaloid biosynthesis was characterized. Moreover, the molecular basis for the catalytic property of the enzyme was elucidated by site-directed mutagenesis and protein modeling (Winzer et al. 2012).