Читать книгу Plant Nucleotide Metabolism - Hiroshi Ashihara - Страница 58

FGAM undergoes ring closure to form 5-aminoimidazole ribonucleotide (AIR) in a reaction that requires ATP (step 5, Figure 4.1, Reaction 5). This step is catalysed by aminoimidazole ribonucleotide synthase (AIRS, EC 6.3.3.1).

The cDNA of pur5 encoding AIRS was identified and isolated from A. thaliana. The functional confirmation of the enzymatic activity was achieved by functional suppression of E. coli auxotrophs using expressed A. thaliana leaf cDNAs (Schnorr et al. 1994; Senecoff and Meagher 1993).