Читать книгу Principles of Virology - Jane Flint, S. Jane Flint - Страница 260

1 You are studying a new DNA virus. You have two cell lines: cell line α expresses the receptor (permissive) but is not susceptible (multiple blocks to the viral reproduction cycle) and cell line β is not permissive but is susceptible. Which of the following statements are correct and why?Transfection of the virus DNA into α will lead to production of infectious particles.Transfection of virus DNA into β will produce infectious particles.Inhibiting production of the receptor in α will allow virus reproduction.Production of the virus receptor in β will allow virus reproduction.

2 You are working on a rhabdovirus that is cytopathic in dog cells but not rodent cells. What strategy would you use to identify the receptor in each cell line?

3 The S (spike) glycoprotein of coronaviruses can engage receptors by the N-terminal domain, the C-terminal domain, or both. The C-terminal domains of both human SARS coronaviruses bind ACE2 (angiotensin-converting enzyme 2) whereas that of MERS-CoV binds DDP4 (dipeptidyl peptidase 4). In contrast, in mouse hepatitis coronavirus (MHV) the N-terminal domain of S binds CEACAM (cell adhesion molecule 1). You have identified a novel coronavirus with an S protein that displays similarity to MHV at the N terminus and to SARS at the C terminus. What are the molecules most likely to function as receptors and how will you test this?

4 Viral fusion proteins mediate fusion between the viral and cellular membranes. This process begins with the insertion of fusion peptides or loops into the target cellular membrane; however, this step alone is not sufficient to complete the fusion process. Describe how additional conformational changes drive fusion.

5 Which of the following statements is correct?Non-enveloped virus entry does not depend on binding to a receptor.For enveloped viruses with multiple envelope proteins on their surface, all envelope proteins engage cell surface receptors.Viral fusion proteins on the surface of enveloped viruses cannot engage a receptor.Fusion of enveloped viruses can occur at the plasma membrane or at compartments of the endosomal pathway.

6 What experiments would you perform to determine whether endosome acidification is required for entry by a particular virus?

7 You produce a viral fusion protein in cells and cocultivate them with cells expressing the receptor. Would you observe cell-to-cell fusion? If you don’t observe this result, what are the possible explanations?

8 Describe the ways in which viral and subviral particles can be transported inside the cell.

9 What are the potential disadvantages for a virion reaching the lysosome?

10 Which of the following pathways would achieve delivery of the viral genome to the nucleus?Delivery of the viral genome to the cytoplasmic side of the nuclear poreHaving a capsid smaller than 39 nmDocking of a partially uncoated capsid to the nuclear pore that does not result in further uncoating or transport through the poreInteraction of the capsid with components of the nuclear pore that leads to subsequent transport and uncoating in the nucleus

11 For retroviruses that rely on cell division to access the host chromatin, how does the viral genome remain in the nucleus once cell division is completed?