Читать книгу Transfusion Medicine - Jeffrey McCullough - Страница 21

In 1908, Moreschi [28] is said to have described the antiglobulin reaction. The potential applicability of this in the detection of human blood groups was not appreciated until 1945, when Coombs, Mourant, and Race [29] published their work on studies of the use of rabbit antibodies against human IgG to detect IgG‐coated red cells. Red cells were incubated with human sera containing antibodies against red cell antigens and washed, and the rabbit anti‐human sera were used to demonstrate the presence of bound IgG by causing agglutination of the red cells. The availability of anti‐human globulin serum made it possible to detect IgG red cell antibodies when the antibody did not cause direct agglutination of the cells. Thus, red cells coated with anti‐IgG red cell antibodies could be easily detected, and the era of antibody screening and crossmatching was born. This greatly improved the safety of blood transfusion and also led to the discovery of many red cell antigens and blood groups.