Читать книгу Open and Toroidal Electrophoresis - Tarso B. Ledur Kist - Страница 10

The main goal of this book is to present electrokinetic based separation techniques in a didactic and concise manner, making the content accessible to the broadest possible audience (students, users, and researchers). In addition, this book provides the exact expressions of separation efficiency, resolution, peak capacity, and many other performance indicators. Appropriate illustrations are presented, and the underlying fundamental phenomena and models are discussed in a logical order instead of in a chronological order. Chemical and physical insights are also developed by emphasizing the microscopic scenarios of the molecular processes involved.

Important mathematical relationships are given to aid users with their practical applications and theoretical studies of the dozens of separation modes performed in the three most common platforms: capillaries, microchips, and slabs. The two most important layouts of these three platforms are also described and compared: the conventional open layout, with an inlet and an outlet for the samples, and the toroidal or closed loop layout, where samples run in a quasi-continuous circulating mode until the desired resolution is obtained.

Motivation to write this book came from twenty years of teaching both undergraduate and graduate students, as well as from stimulating collaborations with so many bright and talented colleagues, to all of whom I am deeply grateful.

The author is thankful to Dr C. Heller, Professor J. H. Z. dos Santos, Professor C. L. Petzhold, Professor A. Manz, Professor Bingcheng Lin, Professor B. Gaš, Professor J. W. Jorgenson, and Professor H-P. Grieneisen for their helpful discussions and suggestions. Additionally, the author is grateful to E. J. M. Bradley for proofreading the manuscript. Finally, it must be mentioned that these people should not be blamed for the errors the author insisted on keeping in this book!

January, 2020

Tarso B. Ledur Kist

Porto Alegre