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2.2.2 Culture Conditions

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The yield of gum was increased in tissue cultures of Acacia nubeculosus by feeding ferrous, calcium, magnesium, and nitrogen contents, but no significant enhancement was recorded by feeding of phosphorus and manganese contents. The addition of Aspergillus flavus (elicitor) alone or in combination with Pseudomonas pseudoalcaligenes increased the yield of gum higher than control (Khalil et al. 2011). In the presence of indole-3-butyric acid (IBA), 2,4-Dichlorophenoxyacetic acid (2,4-D), Murashige and Skoog (MS) culture medium, the calli were formed by shoot explants (Correia and Graça 1995; Yasodha et al. 2004). Proper collection of explants with the right stages of development along with plant growth regulators induce better growth of calli Acacia species (Vengadesan et al. 2002; Quoirin 2003).

Secondary Metabolites of Medicinal Plants

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