Читать книгу Secondary Metabolites of Medicinal Plants - Bharat Singh - Страница 16

Plant cell culture technology is considered as an alternative methodology for the production of secondary metabolites of pharmaceutical importance due to high efficiency and low cost (Rout et al. 2000; Verpoorte et al. 2002; Karuppusamy 2009; Filova 2014). Maximum production of callus was achieved from a combination of 2,4-D and 1-naphthalacetic acid (NAA) for estimation of secondary metabolites (Sen et al. 2014). The cell of A. bidentata, when grown in MS medium with supplementation of NAA and 6-benzyladenine (6-BA), grew rapidly, yielding a maximum quantity of 20-hydroxyecdysone (20E) after 24 days. When cells of A. bidentata were exposed to methyl jasmonate for six days, it was found that total production of 20-hydroxyecdysone (20E) was reached maximum to 2.6-fold higher than control (Wang et al. 2013). The four Agrobacterium rhizogenes strains (MTCC 2364, NCIM 5140, A4, and ATCC 15834) were used for establishment of hairy roots from different explants (young leaf, hypocotyls, cotyledon, and stem segments) of A. aspera. Hairy roots were cultured on the Murashige and Skoog (1962) suspension medium supplemented with 30 g/l sucrose, inducing the highest biomass accumulation and synthesis of phytoecdysteroid 20-hydroxyecdysone (John et al. 2017).