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Graphene is an atom‐thick monolayer of carbon atoms arranged in a two‐dimensional honeycomb structure (Figure 1.1e) (Novoselov et al. 2004). Generally, graphene has been extensively used for a wide array of applications in many fields such as quantum physics, nanoelectronic devices, transparent conductors, energy research, catalysis, etc. (Wang et al. 2011; Huang et al. 2012). However, according to recent technological advancements graphene, graphene oxide, and reduced graphene oxide have shown promising applications in the biomedical field and hence have attracted significant interest worldwide (Gonzalez‐Rodriguez et al. 2019; Yang et al. 2019). Due to its excellent physicochemical and mechanical properties, single‐layered graphene has been widely utilized as a novel nanocarrier for drug and gene delivery in different diseases (Núñez et al. 2018).