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Spectroscopic analyses including Fourier-transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), and mass spectrometry (MS) are used to determine the fundamental functional groups, content and purity of the sample, and the mass-to-charge ratio of ions (molecular weight) of samples, respectively [113, 114]. They help in identifying the modification of functional groups present in the carrier system or identification of conjugated or modified groups of sample under examination undergoing chemical or physical reactions [115–118]. It is more effective in the field of chemical synthesis but also helpful for the qualitative analysis of lipid nanocarrier systems and interactions between lipids, surfactants, and drug compounds.