Читать книгу Biotechnology of Fruit and Nut Crops - Группа авторов - Страница 139

Basal stem rot is the most devastating disease of oil palm in South-east Asia. Tee et al. (2013) examined the transcriptional responses of oil palm roots treated with the causal agent of basal stem rot, G. boninense, using a cDNA microarray approach. A total of 61 from 3748 transcripts examined were found to be significantly up- or downregulated in oil palm roots infected with G. boninense at 3 and 6 weeks postinoculation compared to those from uninfected roots. The differentially expressed genes identified in the artificially infected oil palm roots included genes encoding isoflavone reductase, Em protein H2, SPX domain-containing protein 1, pathogenesis-related protein 1 and vicilin-like antimicrobial peptide. The gene expression of isoflavone reductase, which is involved in the production of phytoalexin and three related genes in the phenylpropanoid biosynthetic pathway, was also profiled in the treated oil palms using real-time quantitative reverse transcription PCR. This information has contributed to our understanding of the defence mechanisms of oil palm in response to infection by G. boninense and future development of molecular markers for marker-assisted breeding and screening of oil palms that are tolerant of basal stem rot.