Читать книгу Biotechnology of Fruit and Nut Crops - Группа авторов - Страница 146

Oil palm protoplasts are suitable starting material for producing oil palm plants with new traits by means of somatic hybridization. Masani et al. (2013) demonstrated the regeneration of viable oil palm plants from protoplasts isolated from suspension cultures. A protoplast yield of 1.14 × 10⁶/g fresh weight with a viability of 82% was achieved by incubating the suspension cultures in a digestion solution consisting of 2% cellulase, 1% pectinase, 0.5% cellulase onuzuka R10, 0.1% pectolyase Y23, 3% KCl, 0.5% CaCl₂ and 3.6% mannitol. The regeneration of plants from protoplasts required medium optimization, the inclusion of plant growth regulators and the correct culture technique. Microcalluses derived from protoplasts were obtained by establishing agarose bead cultures using Y3A medium supplemented with 10 μM naphthalene acetic acid (NAA), 2 μM 2,4-D, 2 μM indole-3-butyric acid (IBA), 2 μM gibberellic acid (GA₃) and 2 μM kinetin. Plantlets were regenerated from microcalluses by somatic embryogenesis after successive subculturing.