Читать книгу The SAGE Encyclopedia of Stem Cell Research - Группа авторов - Страница 138

As compared to the rat or human MSCs, it is a much harder task to isolate MSCs from the bone marrow and proceed for expansion in culture. The murine MSCs (mMSCs) are found to be contaminated with hematopoietic progenitor cells overgrowing in the culture medium when compared to the MSCs from rats or humans. One way to counter this problem could be the removal of hematopoietic precursors by immunodepletion. However, such immunodepletion leads to the accumulation of extracellular matrices in large amounts thereby yielding poor culture expansion. These limitations in the mMSCs’ features have restricted the testing of the cells in important genetic models, such as the transgenic mice. In a recent study, a new method for the isolation of murine MSCs was established wherein the mMSCs so isolated could be extensively expanded. In addition, the cells showed characteristics of multipotentiality and could differentiate into both mineralizing and adipocyte cells. The mMSCs isolated from the inbred mice strains shared the characteristics of rapid proliferation as represented by the single colony forming ability of the low density plated cells when the mMSCs showed a nearly sevenfold increase in growth rate. The cells also differed in their optimal growth requiring media content. The cells showed variation in the surface epitope profiles.