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 Upon arrival, blood samples should stand at room temperature for 30 minutes to sediment the red blood cells. The buffy coat, with lymphocytes, is seen as a thin white layer between the red blood cells and plasma.

 Under sterile conditions, add 1 ml of plasma with the buffy coat to pre‐warmed (37°C) 9 ml cell culture media in a 15 ml centrifuge tube. Mix the tube and incubate for 72 hours at 37°C. Gently invert the cultures twice a day. Note: only blood collected in Na‐ or Li‐heparin Vacutainer® tubes is suitable for cell cultures.

 Start cell harvest at 68 hours of culture following the procedures described under “Cell harvest.”